The present work describes development and validation of a stability-indicating reverse-phase high performance liquid chromatographic (RP-HPLC) method for simultaneous determination of trace level impurities of metoprolol succinate (MET) and hydrochlorothiazide (HCTZ) in their tablets. The stressed degradation study including acid, base, H2O2, thermal and photolytic conditions were performed on MET and HCTZ tablets as per ICH guidelines to prove the stability-indicating capability of the developed method. Degradation was observed in acidic, alkaline, oxidative, thermal condition while no degradation in UV radiation conditions. Chromatographic separation was achieved on Hypersil BDS C18 150*4.6mm column using methanol:water:acetronitrile (60:20:20 v/v/v) using mobile phase. Flow rate was 1.0 ml/min and detection wavelength was carried out at 226 nm. All the known and degradation impurities were separated within 7 minutes. The LOD of MET and HCTZ were found to be 0.34 and 0.10 respectively. The LOQ of MET and HCTZ were found to be 1.03 and 0.31 respectively.
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